CRISPR/Cas – Chances and Challenges

Emmanuelle Charpentier and Jennifer Doudna received the Nobel Prize for Chemistry in 2020 for their discovery in 2011 that CRISPR/Cas can be used to cut DNA at specific sites – a discovery that revolutionized research, especially in the fields of medicine and plant breeding. With this method, it is possible to make specific cuts and modifications to DNA with minimal cost and unparalleled efficiency (Genome Editing). Due to its simple handling, CRISPR/Cas-assisted gene editing is a technology that is further developing very quickly. Alongside other improvements, there are now methods for introducing CRISPR tools directly into plant or animal cells – without the detour via a corresponding gene construct. In essence: we are now in a position not only to read genomes, but also essentially to rewrite them at will.

However, this new method and its further development also raises a number of legal, social, ethical and economic questions, for example regarding regulation or the establishment of an international moratorium on all forms of germ line intervention in humans. Plant breeding is concerned with the question of whether genome editing leads to plants being regarded as genetically modified organisms even though their genomes look "nature-identical". At the same time, the extent to which genome editing can contribute to the sustainable production and distribution of safe food remains highly debated. Another aspect is the problem of patents: who is entitled to which patents for the CRISPR Cas9 technology? And of course there is the question of how best to communicate this technology – its potentials and also its dangers – to a broader audience and initiate a constructive dialog between scientists and the general public.

Spokespersons

• Prof. Dr. Eckhard Wolf (Molekulare Tierzucht und Biotechnologie)

Research Focus Group

• Dr. Monika Aufleger (Institute for the Didactics of Biology, LMU)
• Dr. Sebastian Bultmann (Humanbiologie and Bioimaging)
• Prof. Dr. Klaus Förstemann (Department of Biochemistry)
• Prof. Dr. Christoph Klein (Hauner'sche Kinderklinik und Genzentrum)
• Prof. Dr. Birgit Neuhaus (Institute for the Didactics of Biology, LMU)
• Prof. Dr. Dominik Paquet (Institute for Stroke and Dementia Research (ISD))
• Dr. Silke Robatzek (Biologie, Plant Health)
• Prof. Dr. Nicolai Siegel (Experimental Parasitology)
• Dr. Stefan H. Stricker (SFB 1064 Chromatin Dynamics and Munich Center for Neurosciences – Brain & Mind (MCN), LMU)
• Prof. Dr. Wolfgang Wurst (Munich Center for Neurosciences – Brain & Mind (MCN), LMU and Director of the Institute of Developmental Genetics (IDG), Helmholz Zentrum München)

CAS^Video

Please find video recordings of this Research Focus here: CAS^Video – CRISPR/Cas.

Events

• Panel Discussion within the Lecture Series "Cutting Edge" – "CRISPR/Cas – A New Tool or a Revolutionary Method?"
  (Winter Semester 2020/21)
• Lecture of Prof. Dr. Selim Corbacioglu – "Safety and Efficacy of CRISPR/Cas9 based Gene edited Hematopoietic Stem and Progenitor Cells in Hemoglobinopathies"
  (Summer Semester 2021)
• Lecture of Prof. Virginijus Šikšnys, Ph.D. – "CRISPR/Cas Immune Systems: Expanding the Genome Editing Toolbox"
  (Summer Semester 2021)
• Lecture of Prof. Dr. Holger Puchta – "Applying CRISPR/Cas in Plants: From Gene Editing to Chromosome Engineering"
  (Winter Semester 2021/22)
• Lecture by Prof. Dr. Matthew Porteus – "Genome Editing to Create Personalized Safe and Effective Medicines"
  (Winter Semester 2021/22)